Journal: Frontiers in Microbiology

Article Title: Development of a Novel Lateral Flow Biosensor Combined With Aptamer-Based Isolation: Application for Rapid Detection of Grouper Nervous Necrosis Virus

doi: 10.3389/fmicb.2020.00886

Figure Lengend Snippet: Oligonucleotide sequences used in this study.

Article Snippet: The C-line probe 2 (100 μM, 30 μL) and T-line probe 3 (100 μM, 30 μL) were dispensed on the nitrocellulose membrane simultaneously using a three-dimensional Scribing dispenser (Shanghai Kinbio, China) to form the control line and test line.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Development of a Novel Lateral Flow Biosensor Combined With Aptamer-Based Isolation: Application for Rapid Detection of Grouper Nervous Necrosis Virus

doi: 10.3389/fmicb.2020.00886

Figure Lengend Snippet: Efficient work of the biosensor for RGNNV-CP detection. From left to right are detection of RGNNV-CP with both A-aptamer and C-aptamer, PBS with both A-aptamer and C-aptamer (control 1), RGNNV-CP with only A-aptamer (control 2) and RGNNV-CP with only C-aptamer (control 3). No positive band was observed in the absence of RGNNV-CP, A-aptamer or C-aptamer. Test line could only be observed obviously in the presence of RGNNV-CP (2 μg/mL) and two aptamers. The experiment was performed in triplicates.

Article Snippet: The C-line probe 2 (100 μM, 30 μL) and T-line probe 3 (100 μM, 30 μL) were dispensed on the nitrocellulose membrane simultaneously using a three-dimensional Scribing dispenser (Shanghai Kinbio, China) to form the control line and test line.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Development of a Novel Lateral Flow Biosensor Combined With Aptamer-Based Isolation: Application for Rapid Detection of Grouper Nervous Necrosis Virus

doi: 10.3389/fmicb.2020.00886

Figure Lengend Snippet: Specificity of the biosensor. (A) Results of the biosensor for the detection of RGNNV-CP and control MBP. RGNNV-CP sample can display the visible red line, while MBP did not show a test line. (B) Typical images of biosensor for the lysates detection of RGNNV-infected GB cells, SGIV-infected GS cells, LMBV-infected FHM cells and uninfected cells. Only the lysates of RGNNV-infected GB cells were observed with a distinctive test line. The experiment was performed in triplicates.

Article Snippet: The C-line probe 2 (100 μM, 30 μL) and T-line probe 3 (100 μM, 30 μL) were dispensed on the nitrocellulose membrane simultaneously using a three-dimensional Scribing dispenser (Shanghai Kinbio, China) to form the control line and test line.

Techniques: Infection

Journal: Frontiers in Microbiology

Article Title: Development of a Novel Lateral Flow Biosensor Combined With Aptamer-Based Isolation: Application for Rapid Detection of Grouper Nervous Necrosis Virus

doi: 10.3389/fmicb.2020.00886

Figure Lengend Snippet: Optimization of the biosensor for detection. (A) The biosensor detected RGNNV infection with an incubation time for lysates and aptamers as short as 1 min. (B) The binding time of 5 min for magnetic beads and C-aptamer is needed for the biosensor to detect RGNNV infection. (C) Typical images of biosensor for RGNNV detection with various SDA reaction time. From 10 to 30 min, the level of redness increased gradually. 30 min is enough to get the optimum visible test line. The experiment was performed in triplicates.

Article Snippet: The C-line probe 2 (100 μM, 30 μL) and T-line probe 3 (100 μM, 30 μL) were dispensed on the nitrocellulose membrane simultaneously using a three-dimensional Scribing dispenser (Shanghai Kinbio, China) to form the control line and test line.

Techniques: Infection, Incubation, Binding Assay, Magnetic Beads

Journal: Cell Reports

Article Title: Decision-Making in Cascade Complexes Harboring crRNAs of Altered Length

doi: 10.1016/j.celrep.2019.08.033

Figure Lengend Snippet:

Article Snippet: Probe 3 5′-gcagcctaggttaatatatgacctaaatttgt aggatcac , Metabion , N/A.

Techniques: Virus, Recombinant, Plasmid Preparation, Purification, Software